Flammulina Velutipes Mushroom Farming | Mushroom farming | Biobritte mushroom farming Flammulina Velutipes Mushroom Farming The cultivation of Flammulina velutipes or better known as Enoki or the Winter Mushroom, has a long history, especially in Asia, of its medicinal properties and nutritional values. Step 1: Culture media The first category is the used culture media within these scientific papers. The most used culture media were MEA and PDA, but I also find media like MFM, MMM, and PDYA. Step 2: Substrate The next step towards growing Flammulina velutipes is to find the right substrate mix. Step 3: Sterilization After filling the cropping containers, the substrate is usually autoclaved at 121 °C (250 °F), 15 Psi for 1 hour[26] or if used less pressure at 95 °C (203 °F) for four hours. Step 4: Inoculation Pour the spawn in the hole (bottles) or on top of the substrate (bags). Close the bottles with a lid (with filter) or seal the bag. Step 5: Incubation Some studies go even down to